abYsis

The integrated antibody sequence & structure analysis platform.

Enhance your internal research by installing the very latest version of abYsis behind your firewall.

Install on your own infrastructure, whether it be an internal server or a cloud service and give yourself flexibility.

Analyse protein or DNA sequences interactively or import to store and triage.

  • Try our older public version of abysis for free and explore how it can help your research.

  • Then contact us to learn about the latest version, plus licensing for internal use.

Antibody Numbering

abYsis supports automated antibody numbering and CDR assignment. Kabat, IMGT, Chothia, Martin and AHo numbering are all available in abYsis. Follow the links below for detailed information on the key differences between these most accredited numbering schemes.

Antibody Sequence Alignment

Let abYsis rapidly generate 'antibody aware' multiple sequence alignments for you to analyse.  

Using abYsis allows you to concentrate on your work, rather than how to get bioinformatics algorithms to work.

Position specific residue distributions

Central to abYsis are position-specific amino-acide distributions derived from a statistical analysis of large scale antibody data.

This enables you to identify atypical residues at key positions of your sequences and potentially clusters of such residues that could represent unwanted epitopes that might elicit an anti-antibody response.

Process Your own proprietary data

Input your sequences either through the interactive front-end GUI or through a batch-processing option that can be restricted to power-users. 

Numbering, CDR/framework assignment (Kabat and Chothia), unusual residues and humanness calculations are all automatically applied and await your analysis.

Structural analysis

Back mutation for humanization poses the risk of changing loop conformation. Monitor the predicted conformation as residues are modified.

● Apply canonical structure prediction based on sequence and knowledge of key residue frequencies.

● Map on additional information regarding canonical buried and accessible residues as well as Vh/Vl interactions and determinants.

Humanize

Antibody humanization is one of the most pressing issues in mAb research.

Use our latest interactive tool to analyse any sequence of high interest and set your strategy for humanization. Bring together Germline and Canonical Structure information to investigate in one integrated output.

Display distributions from more than one organism to show differences (as well as similarities) between the source organism (e.g. mouse) and human.

Humanize Tab with Germline and Protein Structure annotation.